1973 Volume 24 Issue 8 Pages 699-703
The present paper provides a new method for a gas-chromatographic analysis of fatty materials, particularly including various polyene acids (or their derivatives), without the use of any tedious additional procedures and discusses the necessary conditions in carrying the method for its practice. The method involves a mathematical treatment of two chromatograms obtained by two separate runs with columns differing in polarity. For the present purpose, the combination of “diethyleneglycol succinate” and “Apiezon grease L” was found to be the most preferable one.
The present method was applied to the analysis of oils obtained from 15 kinds of shell fish by using the stationary phases mentioned above and revealed that there was a considerable difference on the fatty acid composition between the spirals and the bivalves and that the environment of their shell fish had appreciable influence on their oil composition.