Abstract
In regard to inhibition of formation of the strong carcinogenic nitrosamines by L-ascorbic acid, the reaction of sodium nitrite with L-ascorbic acid was studied from their browning reaction.
1. L-ascorbic acid was comparatively fast reacted with sodium nitrite, caused remarkable browning. The degradation of sodium nitrite was very rapid in earlier stage of reaction.
2. The browning reaction products of sodium nitrite-ascorbic acid system was fractionated by Sephadex G-25 column chromatography into two components (F-I, F-II) possessing the UV absorption property and the reducing activity for 2, 6-dichlorophenolindophenol. F-I shows an absorption maximum slightly at 285 nm. F-II shows an absorption maximum at 270 nm. The formation of the browning reaction products was reconfirmed from the results of paper chromatography.
