Oil Absorption from Mouse Stomach

-Histological Study of Fluorescent Substances-

Abstract

Chlorophyl a, vitamin A acetate and Art· 9681 Fluorescin (Merck) emit specific flurescence respectively. In this study these fluorescent substances were used as a marker of oil absorption.
After the ligation of the pyloric region of the mice anesthetized, these fluorescent substances (dissolved in triolein) were given to the mice. Ninety min later, the mice were killed by the dislocation of the cervial vertebrae. Under a fluorescence microscope, many fluorescent minute oil droplets, which were obviously derived from fluorescent substances administered, were observed in the mesogastrium, gastrolienal ligament, thoracic duct, lymphatic vessels of the lung and thymus.
Even in the case of the mouse without the ligation of the pyloric region, many minute oil droplets emitting specific fluorescence were distributed in the mesogastrium and gastrolienal ligament.
Above results prove that triolein could be absorbed from the stomach in the mouse.