抄録
This histopathological study used streptozotocin (STZ)-induced diabetic rats to investigate insulin administration in conjunction with an antibacterial irrigation of chlorhexidine gluconate solution and its effect on the disturbance of periodontal tissue and destruction of alveolar bone induced by mechanical compression at the col due to food impaction. The experimental animals were divided into normal rats (Group N) and STZ-induced diabetes mellitus (DM) rats. Mechanical compression was done by insertion of a gutta-percha point (GP). The diabetic rats were further divided into Group (DM+Ins), in which insulin was administered, and into Group (DM+Chlo), in which 0.2% chlorhexidine gluconate solution was used for bactericidal irrigation. Histological comparisons of the destructive process and reparative changes of the periodontal tissue were performed at 1, 3, 7 and 14 days after the col compression.
The antibacterial irrigation effects caused by chlorhexidine gluconate solution were considered to be low during the early stages. However, since there was no sequestrum formation at the alveolar crest at 7 days after the col compression, reparative changes of periodontal tissue were able to progress. These results suggest that there was a constant antibacterial effect after the chlorhexidine gluconate solution irrigation. Even so, the use of only chlorhexidine gluconate solution in cases with very destructive changes or in cases with persistent mechanical compression, made it difficult to perform a successful treatment of the disturbance of the periodontal tissue. Results for Group (DM+Ins) were better than those for Group (DM+Chlo) with regard to the inhibition of bacterial contaminant at the food impaction area, alleviation, and improvement of the healing process after disturbance in the periodontal tissue. The persistent use of 0.2 % chlorhexidine gluconate solution did appear to lead to a constant effect on the bacteria. Therefore, when treating a disturbance of the periodontal tissue due to food impaction in the diabetic state, the first step should include the administration of insulin in order to improve the diabetic state.
