Oxidative Stress-induced Interaction between Autophagy and Cellular Senescence in Human Keratinocytes

抄録

Oxidative stress in keratinocytes induces cytoprotective events, such as autophagy and cellular senescence. The present study investigated whether an induction of autophagy and cellular senescence can be observed in oxidative-stressed keratinocytes to allow those cells to maintain a cytoprotecitve state. We examined that the effect of various inhibitors on the induction of both autophagy and senescence in H₂O₂-treated HaCaT cells via Western blotting and immunocytochemical assays. H₂O₂-treated cells exhibited increased expression of the senescent markers, p21 and Decades (Dec1), in addition to increased and decreased numbers of senescence-associated β-galactosidase (SA-β-gal) – and Ki-67–positive cells, respectively. These senescent cells also displayed upregulation of the autophagy marker, LC3-II. Attenuation of LC3-II expression using 3-methyladenin inhibited H₂O₂- autophagy and cellular senescence. Our Western blotting results revealed that H₂O₂-induced autophagy was regulated independently by the negative feedback pathway of a mammalian target of rapamycin. By contrast, H₂O₂-induced autophagy and cellular senescence depended on the activation of the p38 mitogen-activated protein kinase α (MAPKα) pathway mediated by the intracellular reactive oxygen species (ROS) production. Furthermore, a suppression of autophagy by 3-methyladenine promoted an induction of apoptosis in H₂O₂-treated cells, suggesting that autophagy, in association with the cellular senescence, may induce the cytoprotection under the oxidative stress. Our findings suggest that the acceleration of both events may allow stressed cells to maintain the cytoprotective effects and may be regulated, in part, by p38 MAPK activation through the intracellular production of ROS.