Role of FGF23 c.35C>A in Bone Remodeling during Orthodontic Tooth Movement

抄録

Fibroblast growth factor 23 (FGF23), which belongs to FGF family, regulates the serum phosphate concentration and plays an essential role in bone development. Our previous study has reported a mutation of FGF23 c.35C>A in the mandibular prognathism (MP) pedigree. The aim of this article was to determine the effect of FGF23 c.35C>A mutation in alveolar bone remodeling during orthodontic tooth movement (OTM). An orthodontic spring was performed in an OTM mouse model for 7 days. Micro-computed tomography (micro-CT) was used to measure the amount of OTM and observe the periodontal ligament (PDL) after mechanical loading, hematoxylin-eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, Runx2 immunostaining. The reverse transcription polymerase chain reaction (RT-qPCR) was also performed to determine mRNA levels. The mutation of FGF23 c.35C>A decreased the OTM, the mRNA expression levels of RANKL, RANK and the count of osteoclasts in compression side (CS). In contrast, the count of osteoblasts in tension side (TS), the mRNA expression levels of COL1A1, OCN were increased when compared to FGF23-WT group after OTM. In summary, we ascertained the obstruction of FGF23 c.35C>A in orthodontic tooth movement, which might due to the stimulation of osteogenesis and the inhibition of bone resorption.