microRNA-324-3p Promotes Osteoblasts Differentiation via Suppressing SMAD7

抄録

Fracture healing is a complex dynamic process that involves the balance between osteoblasts and osteoclasts. Several microRNAs (miRNAs) have been shown to participate in fracture healing. In this study, we investigated the role of miR-324-3p in osteoblast differentiation. MC3T3-E1 cell differentiation was induced by icariin, and miR-324-3p expression levels during cell differentiation were measured using qRT-PCR. Cell proliferation and differentiation were assessed to evaluate the function of miR-324-3p. Luciferase activity was used for target gene verification. During MC3T3-E1 differentiation, miR-324-3p levels gradually increased over time. Further experiments showed that miR-324-3p overexpression significantly promoted cell viability, whereas miR-324-3p downregulation showed the opposite effect. For cells with miR-324-3p mimic, the levels of bone sialoprotein, Runx2, osteocalcin, and alkaline phosphatase activity were significantly elevated. SMAD7 is the target gene of miR-324-3p, and its level is gradually downregulated during MC3T3-E1 cell differentiation. MiR-324-3p may promote MC3T3-E1 cell differentiation by targeting SMAD7.