Isolation of lipid fraction and analysis of fatty acids in biotechnological processing of biomass Rhodobacter Capsulatus

抄録

In the process of the production of photo sensitizer (bacteriophyrphyrin and its derivatives) by the conversation of the biomass Rhodobacter Capsulatus) , extracts of lipids mass amount up to 5 % of dry biomass.The process of production of porphyrinic bacteria from biomass of Rhodobacter Capsulatus also occurs simultaneous with the extraction of lipid components with the preservation of some of their natural native structures.The study of lipids waste has shown, Carotenoids and higher fatty acids as the most dominants components.(On the average 89.1 % of the lipid waste). The remaining components are tochopherol ,quinones and phetol. A double-stage processing of lipid waste was used for a well-directed extraction of the mixture of the components of fatty acids. Fraction of Carotenoid was gotten with the help of desorpting the polar solvents in a periodic process. Fatty acid was been identify on relative time of sustaining components of mixture in comparison to the standard method. The most dominant among Fatty acids based on the result provided by GLC are Oleinic acid (Ń18:1) 92.23%, also identified are fatty acid: C18:0 2.5%; C16:1 2.63 %; C16:0 1.25%; C14:0 1.38 %. Thus, the product of the biomass from Rhodobacter Capsulatus can be as a source of biologically active substances from the class of lipid- Carotenoid and ethers of microbial fatty acids.