肝内結石症のプロテオミクス

抄録

Primary intrahepatic calculosis (IHC), which is characterized by the formation of stones in the hepatic bile duct, is an intractable liver disease and suspected to be one of the causes of cholangiocellular carcinoma. To obtain an insight into the disease, we performed proteomic analysis of the normal and the affected hepatic tissues from patients with primary intrahepatic calculosis by two-dimensional electrophoresis followed by peptide mass fingerprinting. For the normal tissue, 195 spots out of 613 spots were identified as proteins, defining unique 137 proteins. For the IHC tissue, 137 spots out of 671 spots were identified as proteins, defining unique 101 proteins. To further precisely compare, we used two-dimensional difference gel electrophoresis (2-D DIGE). Consequently we identified the up-regulated proteins that were 12 and the down-regulated proteins that were 22. The up-regulated proteins contained the proteins related to plasma and inflammation. The down-regulated proteins contained 60 kDa heat shock protein, annexin VI, glutathione S-transferase A2.