癌初代培養細胞におけるmiRNAとSILACを用いたタンパク質発現解析の関連性

抄録

The initial product of an microRNA (miRNA) gene (>100 bases) undergoes many processing steps in the nucleus, is exported to the cytoplasm as a hairpin and processed by Dicer into single strands. In the presence of the RISC complex, one strand may form an imperfect match with the 3’UTR of an mRNA, which dramatically impedes transcription. Because imperfect base pairing is sufficient, a single miRNA can regulate mRNA across the genome, acting as a Master-of-Ceremony for global events such as proliferation, and motility. Indeed, deregulation of several miRNA genes has been established as a signature of certain cancers. Here we present a correlation between regulatory miRNA abundance in primary malignant breast cells and differential protein expression identified by SILAC.