Fifteen adult dogs were used in this study. The pharyngeal orifice of Eustachian tube was obstructed by laminalia. Epithelium of Eustachian tube was observed in 1 and 3 weeks after surgery using scanning electron microscope. One week later effusion was recognized in the middle ear cavity and the cilia of Eustachian tube lost their metachronal wave. Three weeks later some of the cilia disappeared and others deformed in some parts.