Antibody-secreting hybridoma was generated by fusion of mouse myeloma cells with mouse spleen cells immunized with nasopharyngeal carcinoma (NPC) cell line (NPC 204). Only the monoclonal antibody 5-9 reacted to NPC cells in vitro, and in vivo accumurated to the NPC 204 tumor site, implanted into mice subcutaneously. This monoclonal antibody against NPC cells was conjugated to liposomes containing adriamycin (ADM). And the specificity and antitumor effects of the conjugates were examined in vitro, using NPC 204 cells as the target tumor. The antibody conjugated liposomes were selectively bound to NPC 204 cells. The studies on the cytotoxicity with NPC 204 cells in vitro was clarified that the antibodyconjugated liposomes containing ADM exerted the selective effects on NPC 204 cells in the inhibition assay of the incorporation of 3H-thymidine.
