鼻副鼻腔におけるprotease-抗protease系に関する研究

I. 鼻汁中のfibrinolytic protease-抗protease動態について

抄録

Plasmin is one of the major fibrinolytic proteases and is activated by plasminogen activators and other proteases. It has been reported that there are many plasminogen activators in various tissues (heart, uterus and nasal mucosa) and in the external secretions such as urine and saliva, which are so called secretory activators.
We have studied protease versus antiprotease system and protease inhibitors in nasal secretions which were collected from normal adults and patients with nasal allergy. The activity of total trypsin-like enzyme (amidase) was quantitated by the Eriksson's method and the activities of plasmingen activator and of streptokinase (SK)-reactive activator were determined by the fibrin plate method of Astrup. There was little plasmin, plasminogen and plasminogen activator in nasal secretion of both groups. However, the activity of SK-reactive activator was detected in both groups, and there was significant difference between control and allergy group (p<0.01). There was no relationship between the activity of SK-activator and the amount of albumin. Plasminogen purified from human plasma by lysine-sepharose affinity chromatography was activated to plasmin by urokinase (1000IU/ml). Fraction I of this chromatography was used as inhibitor fraction of the nasal secretion. It was not found that there was inhibitory activity against various proteases (plasmin, papain and trypsin) in the fraction I. Their fibrinolytic activity increased by adding the nasal secretion. Protease inhibitors in both groups were not detected by single radial immunodiffusion. α1-antitrypsin was detected by Ouchterlony's method in 6 of 10 samples from the normal group.
Although abundant existence of plasminogen activator in the nasal mucosa has been reported, we could not detect plasminogen activator and other fibrinolytic activity in the nasal secretion retained in nasal cavity in this study.
It is concluded from these data that fibrinolytic activity has no essential difference between in the nasal secretion of normal group and that of allergy group.