アレルギー性内リンパ腫の実験的研究

抄録

In order to know the validity of allergic theory on Meniere's disease, influence of the antigen-antibody reaction on the guinea pig's labyrinth were investigated. This experiment was performed and confirming that carbon particles injected through the foram. stylomastoideum could reach the labyrinth.
Thirty-one guinea pigs were sensitized by horseradish peroxidase (HRP) for this experiments, and the product of antibody was confirmed by the immunological examination. On the other hand, 17 normal (non-sensitized) guinea pigs were used for the control study.
HRd was challenged through the foram. stylomastoideum to the left labyrinth of all guinea pigs.
In sensitized guinea pigs, horizontal nystagmus appeared to the right side (opposite side) about 15 minutes after challenging, and continued for 24 hours. This nystagmus was not observed in control guinea pigs except injecting dense HRP solution.
Guinea pigs were divided into three groups.
Group I was sacrified at 1 hour after challenging.
Group II was sacrified at 24 hours after challenging.
Group III was sacrified at 48 hours after challenging.
In group I and II, endolymphatic hydrops was not observed. But, particles of HRP were detected in the basement membrane of Reissner's membrane and marginal cells of the stria vascularis by electromicroscopy.
On the other hand, group III has already lost these HRP particles, but endolymphatic hydrops could be observed.
These findings were not observed in the control study, and suggested that the place of antigen-antibody reaction would be Reissner's membrane and stria vascularis. It was thought that hyperpermeability of Reissner's membrane and hypersecretion of the stria vascularis induced by the antigen-antibody reaction were probable cause of endolymphatic hydrops.