DOUBLE IMMUNOENZYMATIC AND TWO-COLOR FLOW CYTOMETRIC ANALYSES OF TONSILLAR T-CELL SUBSETS

YOH RYAN; YASUAKI HARABUCHI; AKIKATSU KATAURA

doi:10.3950/jibiinkoka.93.1869

酵素抗体二重染色法とtwo-color flow cytometryによる扁桃T細胞サブセットの解析

梁耀, 原渕保明, 形浦昭克

著者情報

キーワード: 扁桃, T細胞, 酵素抗体二重染色法, 単クローン抗体

ジャーナルフリー

1990 年 93 巻 11 号 p. 1869-1873

DOI https://doi.org/10.3950/jibiinkoka.93.1869

詳細

発行日: 1990/07/20 受付日: - J-STAGE公開日: 2008/03/19 受理日: - 早期公開日: - 改訂日: -
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訂正日: 2008/03/19 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2008/03/19 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : Distribution of various T-cell subsets in the palatine tonsil was investigated by two-color flow cytometry and double immunoenzymatic stain. Tonsillar lymphocytes contained many (about 20 %) helper T (CD4+Leu8-) cells and few (only 1%) suppressor T (CD8+CDllb+) cells. In interfollicular area, each T-cell subset, i, e., helper, helper-inducer (CD4+CD29+), suppressor -inducer (CD4+CD45RA+), cytotoxic (CD8+CDllb-), and suppressor, was identified by double immunoenzymatic labeling technique using alkaline phosphatase and peroxidase. On the other hand, only two T-cell subsets, helper, and cytotoxic T-cell subpopulations, were recognized in germial center.These results indicate that double immunoenzymatic stain as well as two-color flow cytometry gives us useful informations in terms of tonsillar T-cell subsets.
Right : Distribution of various T-cell subsets in the palatine tonsil was investigated by two-color flow cytometry and double immunoenzymatic stain. Tonsillar lymphocytes contained many (about 20%) helper T (CD4+Leu8-) cells and few (only 1%) suppressor T (CD8+CDllb+) cells. In interfollicular area, each T-cell subset, i, e., helper, helper-inducer (CD4+CD29+), suppressor-inducer (CD4+CD45RA+), cytotoxic (CD8+CDllb-), and suppressor, was identified by double immunoenzymatic labeling technique using alkaline phosphatase and peroxidase. On the other hand, only two T-cell subsets, helper, and cytotoxic T-cell subpopulations, were recognized in germial center. These results indicate that double immunoenzymatic stain as well as two-color flow cytometry gives us useful informations in terms of tonsillar T-cell subsets.

訂正日: 2008/03/19 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Distribution of various T-cell subsets in the palatine tonsil was investigated by two-color flow cytometry and double immunoenzymatic stain. Tonsillar lymphocytes contained many (about 20%) helper T (CD4+Leu8-) cells and few (only 1%) suppressor T (CD8+CDllb+) cells. In interfollicular area, each T-cell subset, i, e., helper, helper-inducer (CD4+CD29+), suppressor-inducer (CD4+CD45RA+), cytotoxic (CD8+CDllb-), and suppressor, was identified by double immunoenzymatic labeling technique using alkaline phosphatase and peroxidase. On the other hand, only two T-cell subsets, helper, and cytotoxic T-cell subpopulations, were recognized in germial center. These results indicate that double immunoenzymatic stain as well as two-color flow cytometry gives us useful informations in terms of tonsillar T-cell subsets.

責任著者(Corresponding author)

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