抄録
Thirty clones were derived by plaque assay on FRI-Splm 1229 cells from a wild isolate of Hyphantria cunea nucleopolyhedrovirus (HycuNPV). Analysis with restriction endonucleases (RENs) revealed that 29 clones out of 30 were distinct in their REN patterns, suggesting a very high degree of naturally occurring genotypic variation. Budded virion (BV) yields of 10 clones were examined in FRI-Splm 1229 cells at 48 and 72 h postinfection (pi), showing that productivity of BV and polyhedrin in the cell culture was different among clones. Two clones with higher BV productivity and two clones with lower BV productivity were each selected and examined for the virulence in per os inoculated H. cunea larvae as well as for the production of BV and polyhedrin in the cell culture. The virulence was different among selected clones, although no clear correlation was made between the virulence for H. cunea larvae and the productivity of BVs and/or polyhedrin in the cell culture. These results indicate that the wild isolate of HycuNPV consists of diverged variants that are distinct in genotype and biological activity.
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