3-3 水熱前処理における自己加水分解の促進による高効率酵素糖化

抄録

In order to improve the economical and energetic efficiencies of glucose production from eucalyptus, the effect of solid concentration during the hydrothermal pretreatment on the subsequent enzymatic production of glucose was investigated. The hydrothermal pretreatment was conducted at 200 °C with various solid concentrations (9.1–50.0 wt%). The subsequent enzymatic hydrolysis was performed at 50 °C for 72 h. It was found that the glucose yield increased with increasing solid concentration during the hydrothermal pretreatment. In the case of concentration of 50.0 wt%, especially, the glucose yield reached to 91.1%. The concentration of acetic acid generated during hydrothermal pretreatment increased with increasing the solid concentration. It is thus considered that the efficient removal of hemicellulose was achieved in high solid concentration because acetic acid accelerates decomposition of hemicellulose by autohydrolysis. Consequently, enzyme access to the cellulose surface during enzymatic hydrolysis should be improved and the high glucose yield was eventually observed.