P-225 バイオディーゼル廃液からの生物的水素・エタノール生産(ポスター発表)

抄録

Glycerol is produced as main by-product in the process of biodiesel fuel (BDF) production. In the near future, the increase of BDF production will cause worldwide surplus of glycerol. Therefore, there is a demand for effective methods of conversion glycerol to more valuable material. We have developed the system of bioconversion of glycerol to ethanol, hydrogen gas and electricity using Enterobacter aerogenes NBRC 12010. The glycerol consumption and products in the hydrogen fermentation of E. aerogenes were affected by the initial glycerol concentration. In bioelectrochemical cells using thionine, and electron transfer mediator between bacterial cells and the electrode, glycerol consumption was kept and more hydrogen and ethanol were produced. Under such conditions, glycerol dehydrogenase kept to activate to consume glycerol. When distilled glycerol solution charged from transesterification of virgin palm oil was used, the amount of glycerol consumed was about 300 mM.