Effective process monitoring techniques are crucial in order to ensure stable and successful reactor operation. In previous studies, many researchers have analyzed fluorescence from F420 in methanogen as an intrinsic probe for monitoring the state of the anaerobic digestion. On the other hand, there is no study for measuring all fluorescence characteristics from fluorescent materials in the anaerobic digester. However, intensity of fluorescence emitted from materials other than F420, or relationship between them may be effective as a new probe. Therefore, Excitation-emission matrices (EEM) of anaerobically digested sludge under wet and dry conditions were measured by spectrophotofluorometer (F-4500, Hitachi) to compare fluorescence spectroscopic characterization with them.
