Rayleigh scattering behavior of asphaltene dispersed in toluene and a mixed solvent of 10% pentane in toluene (10-100 mg/L) was monitored using a multi channel photo detector (λ = 500-1000 nm). Based on Rayleigh law a relationship between observable radius and molecular weight of asphaltene, r^6/Mw, was obtained. The maximum was observed at about 50 mg/L, coinciding with previously reported nano-aggregate concentration of asphaltene.