Experimental study on the holding power of anchors was performed to establish its estimation method. For the experiment, JIS-stockless anchors 5, 15 and 25kg in weight, were used respectively. As a result, it is shown that the holding power of anchor in muddy soil can be expressed by H=C_D ・c・A where H is the holding power of anchor, C_D is the coefficient of resistance, c is shearing strength and A is the vertical projected area of anchor. By using the formula above, the holding power of anchor on the sediments of the main bays in Japan were estimated. The results showed that the formula can be feasible to estimate the holding power of anchor in muddy soil.