オリーブアナアキゾウムシ（コウチュウ目：ゾウムシ科）の人工飼料を用いた室内累代飼育および発育特性

抄録

The olive weevil, Pimelocerus perforatus（Roelofs）, is endemic to Japan and feeds on trees of Oleaceae. Although this species is a serious pest of olive, its ecological and behavioral traits have not been intensively studied because of a lack of continuous laboratory rearing techniques. In this study, we established a continuous rearing method for P. perforatus using an artificial diet for larvae and olive twigs for adults. Adult females were reared with olive twigs and they oviposited into moistened cotton plugs wrapped at the base of the twigs. The eggs were placed on moistened cotton plugs in microtubes until hatching. Larvae were individually reared in Petri dishes and fed an artificial diet of “Insecta F-II” or “Insecta LFS” until pupation, and then the pupae were transferred to other Petri dishes with a moistened Kimwipe. The pupal and total survival rates were higher when the weevils were reared with Insecta LFS than with Insecta F-II. In addition, the larval duration of the weevils fed Insecta LFS was shorter than that of the weevils fed Insecta F-II. Eggs were reared at 20, 25, 27, and 30°C to calculate the developmental zero and thermal constant. Larvae and pupae were reared at 25°C, and the developmental zero and thermal constant were estimated using the present results and data obtained from a previous study at 20, 27, and 30°C. In addition, adult prefeeding, precopulatory, and preoviposition duration were surveyed at 25°C.