The abundances of herbivorous, canopy-visiting, and predatory and parasitic insects were compared between injected and untreated trees to investigate the detrimental effects of dinotefuran trunk injection, used to control beech sawflies, on forest ecosystems. The numbers of Syneta adamsi Baly （Coleoptera: Chrysomelidae） and lepidopteran larvae, the proportion of leaves infested with Hartigiola faggalli （Monzen） （Diptera: Cecidomyiidae） galls and those deformed by Phyllaphis fagifoliae Takahashi （Hemiptera: Aphididae）, and the proportion of acorns with traces of insect-feeding were significantly lower in injected trees than in untreated trees. However, no significant differences were observed between treatments regarding the number of plant-feeding insects, such as Aphthona perminuta Baly （Coleoptera: Chrysomelidae）, the proportion of leaves with galls formed by Cecidomyiidae sp., those infested with Platyaphis fagi Takahashi （Hemiptera: Aphididae）, or the number of canopy-visiting insects, such as hemipteran, dipteran, and lepidopteran adults. The numbers of hymenopteran parasitoids and small- （≤1 cm） and medium-to-large-sized （＞1 cm） predatory cantharid beetles were lower in injected trees than in untreated trees, which could be attributed to an indirect effect due to the number of prey. These findings revealed that the effects of dinotefuran trunk injection were limited to insects that fed on leaves and seeds.

To investigate the occurrence of Leptocorisa chinensis in its northernmost distribution area, we conducted net-sweeping surveys in the coastal region of Iwate Prefecture, Japan, from 2020 to 2023. These surveys were performed annually in early and mid-September, coinciding with the period when overwintering adults have almost completed their dispersal and expansion of habitat range and first-generation adults start to emerge. We sampled 12 to 20 locations in grassy fields dominated by poaceous plants, especially Echinochloa crus-galli（L.）P. Beauv. var. crus-galli. Data for L. chinensis were analyzed considering factors from neighboring regions that influence the occurrence and abundance of the species. Our survey indicated that the northernmost boundary of L. chinensis extended 70 km further north than previously reported. However, the analysis of the occurrence of L. chinensis against the average maximum temperature in early February and the distance from Japanese cedar forests （overwintering habitats） did not yield statistically significant results. This discrepancy may be attributed to differences in survey methods and generational variations of L. chinensis during the research period compared to earlier studies. Overall, L. chinensis has shown an expansion in its range, highlighting the need for ongoing monitoring and continuous surveys.

In recent years, the distribution area of Blattella nipponica has expanded northward in Japan. Since this species is a problematic nuisance and sanitary pest, the future expansion of their distribution should be predicted. Therefore, in this study, we investigated the influence of rearing temperature on the larval development. Significant arrested development was observed for this species at temperatures below 22°C, especially in the sixth-instar larvae. Arrested development was observed also at temperatures above 32°C, possibly due to high-temperature damage. Therefore, we calculate the developmental zero excluding data obtained at temperatures higher than 30°C. As a result, we could calculate the developmental zero and effective accumulated temperature for all instars, we estimated the environmental conditions in which this species can develop into an overwintering stage before the arrival of winter. The results suggested that the northern limit area of this species is Ibaraki Prefecture, but in fact this species has been found in Fukushima Prefecture. This may be because the Shimane population was used for this study. A more accurate estimate could be made by using a more northern population and by clarifying the life history of the northern population.

Orius strigicollis （Poppius） often establishes poorly after release, especially during periods of low prey density. Artificial selection for reduced walking activity extends the patch residence time and improves the early establishment of O. strigicollis. However, long-term artificial selection can affect the life history characteristics through inbreeding and/or correlated response to artificial selection. The effects of artificial selection for reduced walking activity on the hatching rate, proportion of emergence, developmental period, and early fecundity were assessed by comparing selected and wild-type lines of O. strigicollis under laboratory conditions. No differences were found in these characteristics. In contrast, with no diet provided, O. strigicollis adults from the selected lines survived significantly longer than wild-type adults. These results suggest that artificial selection for reduced walking activity does not influence the survival, developmental, or reproductive characteristics deleteriously and improves resistance to starvation in O. strigicollis.

Cryopreservation techniques have the potential to strengthen the ex-situ conservation of insects. The cryopreservation technique developed for the ovaries of Bombyx mori Linnaeus can be applied to ex-situ conservation of endangered species. This technique involves the surgical process of transplanting cryopreserved ovaries to recipient larvae. However, the technique should be adjusted to each taxonomic group or species. Humidity is a factor that should be considered during the post-surgery period because castration or transplantation surgeries of insects are often conducted under high humidity conditions. The present study investigated the effect of humidity on the survival rate of the castrated larvae of a pierid butterfly, Eurema mandarina（de l’Orza）（Lepidoptera: Pieridae）. The result of model selection showed that there was little difference in the emergence rate of adult individuals reared under 60 and 100% relative humidity（RH）a day after castration, indicating that the method has the potential to be used without humidity control.