Japanese Journal of Biological Education
Online ISSN : 2434-1916
Print ISSN : 0287-119X
RESEARCH PAPER
Experiments on Immunology in Senior High School Biology using the Antibody on the Market
―the observation of antigen-antibody reaction using the Ouchterlony method―
A. Motohashi
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2012 Volume 53 Issue 1-2 Pages 1-9

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Abstract

There are not many examples of experiments on immunology in senior high school biology classes. Therefore the experiments on immunology to observe the antigen-antibody reaction with the Ouchterlony method using rabbit antiserum against bovine serum albumin on the market, were worked out. In basic biology experiments, students can actually see the antigen-antibody complex with their own eyes and further recognize that antibodies do not crossreact with self components and that antibodies are made by an injection of antigen. In addition to these, students can observe, in biology experiments, the difference of antigenecity among serum albumin in several animals and it is possible for them to immunologically surmise phylogeny of these molecules.

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© 2012 The Society of Biological Science Education of Japan
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