新開発根管充塡剤のヒト歯根膜線維芽細胞様細胞の及ぼす影響について

抄録

  A comparison of the effects of newly-developed root canal sealer and commercially available sealers of a cultured human periodontal ligament fibroblastic (HPLF) cells was done in this study. Various root canal sealers were applied to cultured HPLF cells obtained from premolars extracted for orthodontic reasons. Measurement of [H³]-thymidine incorporation and alkaline phosphatase activity was carried out after 24 and 48 hrs.. No significant difference was observed in cellualar DNA synthesis and alkaline phosphatase activity of cells exposed to No.1, No.5, No.6, Canals-N and controls at 24 and 48 hrs.. Canals and Diaket significantly inhibited DNA synthesis and alkaline phosphatase activity at 24 and 48 hrs.. Sealapex had no inhibitory effect on DNA synthesis and alkaline phosphatase activity at 24 hrs., but Sealapex showed inhibitory effect at 48 hrs.. Newly-developed root cadal sealers demonstrated lower toxicity than canals and Diaket in vitro.