Public Hygienic Study of replacemented A type-zeolite by Ag, Zn, NH₃ Part II : Dermatological Test

Abstract

In the Law Concerning the Examination and Regulation of Manufacture, etc. of Chemical Substances (Chemical Substances Control Law), there is a demand for data on environmental pollution and dermatological tests for novel chemical substances. Considering the many existing examples of chemical substances, the frequency of irritation can be considered to be samll. However, up to now testing has been for chemical substance being developed. The sample of reference is an existing chemical substance. However, it is a substance where Ag, Zn, and NH3 have been bonded into an A-type zeolite synthesized by an ion-exchange reaction and is not a substance that could not be chemically considered to be a new chemical substance. This substance is to be used by mixing it with clothing for hospitals and other locations where cleanliness is a necessity. Also when considering sanitary clothing for infants, it is necessary to establish data on skin irritation as part of the public hygienic research. The primary dermatological test for AZN zeolite was performed following the Draize method in accordance with OECD guidelines. 0.5g or 0.05g of AZN zeolite was spread on the back skin (2.5x2.5cm) of white New Zealand strain rabbits and left for 24 hours. After removal, skin changes were macroscopically observed for 0.5, 24, and 48 hours. No skin change was observed for both the 0.5g and 0.05g dosages of AZN zeolite on normal and comeal abrasion skin parts. From these results, the primary irritation index (PII) was set at 0 based on the eveluation at 0.5 and 48 hours aftere the AZN zeolite was removed and, the irritation of skin was concluded to be almost absent.