Abstract
Real-time PCR method and ELISA method for quantitative determination of genetically modified soybean (CMS) and de-fatted soybean were compared. CMS contents in mixed soybean grain samples, IRMM standard, ELISA standard and IP handling soybean were determined using real-time PCR method and ELISA method. In soybean grain samples, a high correlation between GMS contents determined using real-time PCR method and ELISA method was observed. On the other hand, no correlation was observed between the GMS contents (%) determined using real-time PCR method and ELISA method in de-fatted soybean samples. These results suggested that both real-time PCR method and ELISA method would be applicable for the determination of GMS contents in soybean grain samples, but the values determined using real-time PCR method seem to be higher than those using ELISA method in de-fatted soybean samples.
