Abstract
An ELISA detection system specific for neomycin phosphotransferase II (NPTII) protein was constructed using the antibodies against overexpressed NPTII protein in recombinant Escherichia coli. NPTII protein in genetically modified (GM) tobacco plants could be qualitatively detected using this system; however, non-specific detection in normal tobacco, soybean, corn and potato was not observed. These results suggest that the constructed ELISA system can monitor NPTII protein in GM foods.
