2010 Volume 17 Issue 3 Pages 231-235
A simple and rapid high-performance liquid chromatography (HPLC) method was developed for the analysis of trans-10-hydroxy-2-decenoic acid (10-HDA) in royal jelly (RJ) products. In sample preparation, 10-HDA in RJ was extracted with methanol, and supernatant obtained by centrifugation was used as HPLC test solution. The HPLC separation was achieved on a reversed-phase C18 column using an isocratic mobile phase of 0.6% phosphoric acid- methanol (1:1, v/v) at a flow rate of 0.8mL/min, followed by ultraviolet (UV) detection at 215nm. The detector response for 10-HDA was linear (r>0.999) in the concentration range 1-100μg/mL. Spiked 10-HDA recovery for tablet, liquid drink and raw material were 93.0-110.4%. The method was applied to 21 RJ commercial products. The concentration of 10-HDA was 0.2-45.8mg/g, and the ratio of calculated RJ contents to labeled value was 58.1-94.6%.
