Chemical constituents of Gentoku-cha (Japanese post-fermented tea) were determined by HPLC and HPLC-TOFMS analyses. Gallic acid, (+)-catechin, flavonoids (quercetin and kaempferol), teadenols (teadenol A and teadenol B), teasperol and teasperin were identified in the tea. In Gentoku-cha, flavonoids content was relatively larger than that of catechins.