Evaluation of anti-inflammatory properties of hot water extract from Coprinus comatus using HL-60 and 3T3-L1 cells

Abstract

In this study, the amount of ergothioneine (EGT) in hot water extracts of Coprinus comatus (Coprino extracts) was studied, and the anti-inflammatory activities of the extracts were evaluated using a cell system by simultaneously monitoring superoxide anion radical generation and intracellular Ca²⁺ levels in neutrophil-like HL-60 cells. The protective roles of the Coprino extracts and EGT against inflammatory oxidative damage of 3T3-L1 adipocytes induced by tumor necrosis factor alpha (TNF-α) were also examined. Based on these results, it is reasonably safe to conclude that Coprino extracts have a higher anti-inflammatory activity than EGT alone.