Abstract
A simple and rapid dialysis method was developed for the analysis of sweeteners such as aspartame, sucralose, dulcin,
acesulfame potassium, sodium saccharin, and sodium cyclamate present in foods. In the conventional method, a suspension was
prepared by mixing 20 g of food with the dialysate and added to the dialysis membrane; the final volume was made up to 200 mL
with the addition of the dialysate. The dialysis continued for 24-48 hours, after which the sweeteners that permeated the dialysis
membrane were analyzed by high performance liquid chromatography. In the development method, a suspension was prepared by
mixing 5 g of food with the dialysate and a dialysis membrane containing 2 mL of the dialysate was added; the final volume made
up 50 mL with the addition of the dialysate. The dialysate comprised of 30% methanol containing 0.5% ammonium formate.
After 90 minutes, the inner solution of the dialysis membrane was taken and analyzed by the liquid chromatograph-photodiode
array-mass spectrometry. Using the above protocol, a recovery rate from 88.8 to 109.4 % and a % RSD from 0.2 to 4.8 was
obtained.
