Abstract
Most edible mushrooms are grown in mushroom beds, but reusing the waste beds after cultivation is difficult, and they are often discarded. Therefore, developing a use for waste beds would be beneficial. The waste beds after mushroom cultivation contain syringic acid (SA) and vanillic acid (VA), which are phenolic compounds with anti-inflammatory and antioxidant activities. This study quantified SA and VA in mushroom beds cultivating Lentinula edodes (KX-S055 and XR-1) and Hypsizygus marmoreus (KX-BS023 and KX-BS025). The SA and VA amounts in the mushroom beds gradually increased after inoculation, indicating that SA and VA were produced during mushroom cultivation. In vitro, SA and VA were produced from the reaction between the wood component, lignin, and the waste bed protease extracts. These results suggest that SA and VA were produced by lignin degrading from mushroom proteases.
https://ror.org/012eh0r35 
