Abstract
With the aim of disseminating antibiotic analysis methods to medical professionals in the region, technical training of the analytical method of oxytetracycline (OTC) utilized as medicine and pesticide was provided to medical students in Osaka Prefecture. The residual OTC was extracted using a McIlvaine buffer containing EDTA-2Na. The solid-phase column, InertSep PLS-2, was employed to remove the matrix from the extract. Following the removal of the elution solvent from the solid-phase column, the extract was dissolved in a potassium phosphate solution. The concentration of OTC in the test solution was determined quantitatively using HPLC with a photodiode array detector (PDA). Recovery tests were conducted to validate the analytical method at 0.2 μg/g to evaluate the trueness, repeatability (RSDr), and within-laboratory reproducibility (RSDwr) by medical students. The validation results for three pear beverages exhibited sufficient trueness (79~90%) and precision (RSDr ≦ 5% and RSDwr ≦ 7%). This training provided medical students with an in-depth understanding of antibiotic analysis.
