抄録
Studies were made on the egg-yolk reaction of leptospira and also on hemolytic factor produced in the reaction mixture.
The hemolytic factor is different from the themolabile hemolysin reported by Alexander in 1956 in that its hemolytic ability on rabbit erythrocytes was almost the same as on sheep erythrocytes.
The saprophtic strains showed generally strong positive reaction and strains belonging to pahtogenic serotypes showed variable results. Among strains of L. icterohaemorrhagiae, strain Mikawashima was very active and on the other hand strain Asakawa was not so.
The egg-yolk factor participating in the reaction was precipitated from culture fluid at 50% ammonium sulfate saturation and lyophilyzed after dialysis. The lyophilyzed powder was resolved in saline and then used for further studies. It was observed that Ca++ and Mg++ had stimulatory effects on the reaction.
