Abstract
We carried out directly microtomy to prepare samples for PIXE (Particle induced X-ray emission) analysis. The microtomies of biological material were sectioned at 20μm thickness. PIXE analysis of these samples gave result that there were less than 10% coefficients of variation of the elemental intensities for major elements (chlorine, phosphorus, sulfur, potassium, etc.) in biological material. Elemental composition values obtained, therefore, are regarded as representation value of an object organ. Furthermore it was shown that the method of microtomy is effective for halogen elements. Elemental composition values obtained with our method and those with INAA (Instrumental neutron activation analysis) do not agree well because of difference of these preparations. Our preparation method for PIXE analysis is regarded as microstructure to direction of microtomy layer, while the preparation of INAA is more macrostructure than that of PIXE. Because of this reason, PIXE and INAA are complementary each other, and employment of both methods increases analysing power for biological material.
