mtDNA sequence of the Acheilognathus tabira jordani lineage in the Hokuriku Region, Toyama Prefecture, Japan

Abstract

Environmental DNA (eDNA) techniques are being used increasingly in fish faunal surveys, although misidentifications may still occur due to, for example, incomplete databases. Although an Acheilognathus tabira subspecies has been reported from the Hokuriku region (Toyama Prefecture, Japan) as A. t. jordani, a recent eDNA survey in the area also found A. t. tohokuensis. The present genetic analysis of A. t. jordani collected from the Hokuriku region revealed genetic characteristics that differed from those of A. t. jordani from the Sanin region (southwest Honshu), according to the database used for species determination in the eDNA survey. It was concluded that A. t. tohokuensis previously reported in the eDNA survey was likely have been a misidentification due to database limitations.