Biochemical and Immunological Studies on Aspergillus

I. Chemical and Biological Investigations of Lipopolysaccharide, Protein and Polysaccharide Fractions isolated from Aspergillus fumigatus

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Lipopolysaccharide, protein and polysaccharide fractions were extracted and purified from A. fumigatus. Pyrogenic activity for rabbits was found by the intravenous injection of lipopolysaccharide at 0.125 mg doses.
The protein fraction was active in delayed type skin reaction at 10 μg in the rabbits of experimental aspergillosis. Purified protein fraction (AAP-A) contained 13.48 per cent of nitrogen and a small amount of sugar.
Two kinds of polysaccharide, APS-33 and APS-66, were extracted and purified by the fractional precipitation with acetone, column chromatography on Dowex 50 and DEAE cellulose column and gel filtration. Precipitation antigenicity was shown in APS-66 using antiserum of the rabbits of experimental aspergillosis. The skin-test antigenicity was not found in both purified polysaccharide fractions. By the gas-liquid chromatographic analysis of sugar component, it was shown that APS-33 was composed of glucose only and APS-66 was composed of galactose and mannose. The molecular weight of both polysaccharides was larger than 50, 000.