Actinomyces viscosusによるヒト末梢血リンパ球の活性化についての研究

抄録

The supernatant of sonicated Actinomyces viscosus (AVsup) and Actinomyces Israelii (Alsup) were examined for their mitogenecity to human peripheral blood lymphocytes.
Peripheral blood mononuclear cells were separated into three groups: 1) mononuclear cell (unseparated) fraction, 2) T cell enrich (E-receptor positive cell) fraction, 3) B cell enrich (E-receptor negative cell) fraction.Each cell fraction was cultured with AI and AVsup respectively for 72 hrs and DNA synthesis was assayed. Streptococcal preparation (OK-432) and PWM were also tested as positive controls.
AVsup induced a low increase of DNA synthesis on T cell enrich and B cell enrich fraction, but a higher increase on mononuclear cell fraction. Alsup did not induce any increase of DNA synthesis. OK-432 and PWM induced higher DNA synthesis on T cell enrich fraction as compared with B cell enrich fraction, and on mononuclear cell fraction, DNA synthesis was induced higher than T cell enrich or B cell enrich fraction. Furthermore, the change of T cell subsets were observed using FITC conjugated monoclonal antibodies at 24, 48, and 72 hrs cultures.AVsup did not induced increase of OKT3 (T cell), OKT4 (helper/inducer T cell), OKT8 (suppressor/cytotoxic T cell) antigen positive cell population.However, it increased OKDR antigen positive cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it failed to increase OKB7 (95% of B cells) antigen positive cell population.So the increasing of OK DR antigen positive cell population was considered not as B cells but activated T cells.
It might be strongly suggested that AVsup induced T cell activation without being accompanied with remarkable blastogenesis and proliferation, and then the B cell was stimulated by the activated T cell.