ヒト口腔扁平上皮癌における制癌剤感受性試験の基礎的研究

Human Placental Conditioned Mediumのコロニー増殖刺激とその有効成分の解析

抄録

In order to improve human tumor clonogenic assay (HTCA) that is in vitro chemosensitivity test for anticancer drugs, human placental conditioned medium (HPCM) was applied to HTCA using double soft agar system on human oral squamous cell carcinomas (SCC).
Human oral SCC growth on cultured cell lines, surgical specimens and cancer xenografts in nude mice were significantly stimulated by HPCM, so that HPCM was identified as the useful growth factor for human oral SCC.
Furthermore the author tried to analyze the growth promoting factors contained in HPCM. Human chorionic gonadotropin (hCG) and its α and β subunits that were included in HPCM promoted colony formations. In one of four human oral SCC cell lines, the transcription of hCGβ mRNA was identified by Northern blot analysis and hCGβ was stained by immunohistochemical method.
These results suggest that hCG or hCG like substances in HPCM can promote in vitro growth of human oral SOC.