微粒子活性炭吸着ペプロマイシンとOK-432のリンパ節転移抑制効果に関する実験的研究

抄録

This experimental study was undertaken to investigate the effect of anticancer agent (peplomycin (PEP)) and OK-432 (OK) adsorbed on activated carbon particles (CH) upon lymph node metastasis of VX 2 carcinoma implanted in the tongue of domestic rabbits. The drugs (PEP, PEP-CH, and PEP-OK-CH) were given by topical injections into the tumors of the tongues. The preventive effect on lymph node metastasis by these drugs was determined by investigating the positive rate of metastasis and the occupied area rate by metastatic lesion histopathologically. And electron microscopic observations on metastasis in lymph nodes were performed.
The following results were obtained:
1. The values of the positive rate and the occupied area rate on lymph node metastasis in the PEP-CH and PEP-OK-CH groups were significantly lower than those in the PEP group. But there wasn't any significant difference between the PEP-CH and PEP-OK-CH groups.
2. Histopathological findings on lymph node metastasis in the PEP-OK-CH group revealed the distinct necrosis, and that many lymphocytes invaded around the degenerated VX 2 carcinoma cells.
3. Electron microscopical findings on VX 2 carcinoma cells in the PEP-CH and PEPOK-CH groups showed an increase of tonofilaments, pycnosis, karyorrhexis, and vacuolization as compared with those in the PEP group.
The results of these investigations indicated that topical injections of PEP-CH and PEPOK-CH were effective for preventing lymph node metastasis. Then, it was thought that these drugs would be a useful dosage form for preventing lymphatic metastasis in oral cancer chemotherapy.