電気刺激骨膜とヒドロキシアパタイト併用による実験的顎骨再建

抄録

Transplantation of hydroxyapatite (HAP) or boiled bone (BB) with electrically stimulated periosteum (ESP) was carried out to treat muscle and bony defects of rats and evaluate the potential for application in mandibular reconstruction.
Experiment 1. Male Wister rats, 8-10 weeks old, were used. The tibial periosteum of each rat was electrically stimulated using 15.5, μ A of direct current for 1, 3, 5, 7, 9, and 12 days. The periosteum was removed and transplanted into the abdominal muscle. Rats were then sacrificed on the 5th, 7th, 14th, 28th, and 56th days post-transplantation; soft X-rays were taken and the tissue was examined histologically.
Experiment 2. The tibial periosteum was electrically stimulated for 7 days, and HAP or BB along with the ESP was transplantaed into right mandibular bony defects. Each rat was killed at 3, 5, 7, 14, 28, and 56 days after grafting, and tissue was examined histologically.
The following results were obtained:
1. Transplantation of ESP ploduced by stimulation for 7 days was associated with differentiation into bony tissue in abdominal muscle.
2. No bone tissue was formed around HAP or BB after transplantation of only HAP or BB. Bone tissue was formed after transplantation of HAP or BB with ESP.
3. HAP with ESP or BB with ESP is expected to be a useful material for mandibular reconstruction.