唾液腺腺様嚢胞癌におけるタンパク発現プロファイリング

抄録

To identify specific markers of adenoid cystic carcinoma (ACC), we profiled protein expression in ACC and normal salivary glands (NSGs) by fluorescent 2-dimensional differential in-gel electrophoresis (2-DDIGE), a new technique for comparative proteomics. To identify the proteins, matrix-assisted laser desorption/ionization time-off-flight (MALDI-TOF) peptide mass fingerprinting (PMF) was carried out. Using these strategies, we detected three up-regulated proteins and three down-regulated proteins in ACC. Among these proteins, we obtained two antibodies, which were specific to stathmin and maspin, respectively. With the use of these antibodies, Western blotting confirmed the differential expression of these twoproteins in five clinical samples of ACC tissue, as compared with NSGs. These results may contribute to a better molecular understanding of ACC, as well as to the development of useful clinical biomarkers for tumors.