GTPγS-Induced Ca2+ Activated Cl− Currents: Its Stable Induction by Gqα Overexpression in Xenopus Oocytes

Muneshige Kaibara; Yoshihisa Nagase; Osamu Murasaki; Yasuhito Uezono; Yoshiyuki Doi; Kohtaro Taniyama

doi:10.1254/jjp.86.244

Short Communications

GTPγS-Induced Ca²⁺ Activated Cl⁻ Currents: Its Stable Induction by Gqα Overexpression in Xenopus Oocytes

Muneshige Kaibara, Yoshihisa Nagase, Osamu Murasaki, Yasuhito Uezono, Yoshiyuki Doi, Kohtaro Taniyama

著者情報

キーワード: Gq, GTPγS, oocyte

ジャーナルフリー

2001 年 86 巻 2 号 p. 244-247

DOI https://doi.org/10.1254/jjp.86.244

詳細

抄録

In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPγS) (30 mM, 5 nl) did not induce Cl⁻ current in 11 out of 22 oocytes. Injection of increased concentration of GTPγS (100 mM, 5 nl) into the oocytes induced Cl⁻ currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gqα, GTPγS (30 mM, 5 nl) faithfully evoked Ca²⁺-activated Cl⁻ currents. These results indicate that heterogeneous expression of Gqα in Xenopus oocytes provides a useful system for studying the functional roles of Gqα in regulating cellular events.

責任著者(Corresponding author)

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