抄録
Mandelonitrile in Apricot Kernel Water was studied by high-performance liquid chromatography using an octadecyl bonded stationary phase. According to the Kawai method, 0.5ml of Apricot Kernel Water is treated with 0.3ml of 1% hydroxylamine hydrochloride solution, which react with free benzaldehyde. After an excess of hydroxylamine is removed by adding 0.2ml of acetone, the mixture is treated with 0.3ml of 10% ammonia solution. And then, the aqueous solution is saturated with NaCl, and the benzaldehyde produced corresponding to mandelonitrile is extracted with 2ml of ethl acetate containing 0.6mg of α-(o-tolylazo)-β-naphthylamine as an internal standard. This method was applied to analysis of a colored preparation composed of Apricot Kernel Water, Senega Syrup and M. Verdau J (3: 5: 5) or Apricot Kernel Water, Liquid Brocin and M.Verdau I (3: 5: 10). With this method, the amount of benzaldehyde produced corresponding to mandelonitrile was determined within 1.68% error over the range from 0.5-5.0μg/ml.
