Japanese Journal of Hospital Pharmacy
Online ISSN : 2185-9477
Print ISSN : 0389-9098
ISSN-L : 0389-9098
A Simple Colorimetric Determination of Bromvalerylurea in Acute Toxipathic Patient's Serum
YUKO NAKAMURA, AKIHIRO HARA, YURIKO KONDOU, MIKA MORITA, YUTAROU AZUMA, NORIMASA TAKIZAWA, HIDEKI KAWASAKI, HIDETAKA YUKI
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Keywords: serum bromvalerylurea, colorimetric determination, diacetylmonooxime, acute toxpathic patient
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1989 Volume 15 Issue 1 Pages 55-59

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Abstract
A new method for the determination of bromvalerylurea (BVU) in the serum of acute toxipathic patients was developed.The serum was incubated with urease to hydrolyze urea which interferes with the determination of BVU. After deproteinization with trichloracetic acid, the sample solution was reacted with diacetylmonooxime, and the color developed was measured at 525nm.A straight calibration curve was obtained between 10 and 300μg BVU/ml serum.This method was simple and precise, and was applied to the acute toxipathic patient's serum.
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