抄録
The disinfective effects of both ionic surface active agents sold in Japan were evaluated in antiseptic-resistant strains of methicillin-resistant Staphlococcus aureus. The four agents used in this experiment were TEGO®-51, HYPAL® No.20, HYPAL® No.5, and OBANOL (Mitsumaru). Minimum inhibitory concentrations (MICs) of OBANOL and HYPAL® No.20 were almost the same as all of the strains used by the broth dilution method. The MICs of HYPAL® No.3 and TEGO®-51 were higher than those of OBANOL and HYPAL® No.20. There was little difference in the MICs of the four agents to all strains used in the agar dilution methods. The MIC of OBANOL to antiseptic-high level-resistant strain was the same as that to the sensitive strains.
The effects of the concentration of the agents and the duration of exposure on the survival of the strains were also determined. The antiseptic-high level-resistant strain N20 was killed by OBANOL, HYPAL® No.20, and HYPAL® No.3 at the concentration of 0.031% within 1.5 minutes, but was not killed by TEGO®-51 under the same conditions. The effects of the four agents for disinfecting the hands were additionally evaluated. After 105 cells of bacteria were inoculated on hands, the hands were disinfected with the agents under the accompaning references. OBANOL killed all strains including the high-level antiseptic-resistant strain in three diferent experiments. From these results, the most effective agent for disinfecting against the high-level antiseptic-resistant strain among those agents used in this experiment was OBANOL.
