抄録
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a calcitonin generelated peptide-like immunoreactive substance (CGRP-IS) was developed. In competitive reactions, the CGRP-antibody was incubated with CORP standard (or sample) and β-D-galactosidaselabeled synthetic human αCGRP fragment [residue (8-37)](delayed addition). Free and antibody -bound enzyme haptens were separated by using an anti-rabbit IgG coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows the detection of 2.1 to 19.5 fmol/mL of CORP. Using the proposed EIA, CGRP-ISs in human saliva and plasma were determined.
The levels of CGRP-IS in human saliva were about 3.2 fmol/mL, which were much higher than that in human plasma.
