抄録
A new immuno precipitation inhibition technique (i-PiT method) was successfully applied to the determination of phenobarbital (PB) and phenytoin (PHT) in plasma. Each coefficient of variation for the 3 levels of PB or PHT was less than 10% within-run and between-run. 82 plasma specimens of epileptic patients were examined by the i-PiT method and absorptiometry. There was good agreement between the i-PiT and absorptiometry results, and the coefficients of correlation were 0.990 for PB and 0.995 for PHT.
Neither primidone and carbamazepine, nor the metabolite of PB or PHT, p-hydroxyphenobarbital or p-hydroxyphenylphenylhydantoin, interfered the i-PiT method under therapeutic conditions. Therefore, the i-PiT method will be useful for the plasma level monitoring to patients undergoing PB and PHT therapy.
