THE ORGANO-IRON COMPOUND IN THE DIGITONIN EXTRACT OF THE ROD OUTERSEGMENT
1962 年 12 巻 5 号 p. 460-466_1
詳細
1962 年 12 巻 5 号 p. 460-466_1
1 Iron ion was found in an apparently pure rhodopsin solution, which was extracted from the outersegments of rods of frog retinae.
2. No demonstration was made of the existence of cytochrome oxidase and peroxidase in the outersegments of rods of frog retinae with Nadi and benzidine reagents respectively.
3. The organo-iron compound contained in rhodopsin solution was differentiated from heme-proteins such as hemoglobin, cytochrome c and catalase, by a separation technique with a water-alcohol-chloroform mixture.
4. A rhodopsin molecule seemed to contain at least one iron ion.
5. Discussion was made of a possibility that the λmax at 500 mμ of the absorption spectrum of rhodopsin was attributable to iron ion.
The author wishes to thank Prof. Y. HOSOYA and Prof. E. KIMURA for their helpful suggestions and criticism during in finishing the present study.
